| 5-氮杂-2＇-脱氧胞苷对卵巢癌细胞系CP70顺铂耐药性的逆转作用 |
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| 引用本文： | 张琳琳,辛晓燕,张建芳,白露,高静. 5-氮杂-2＇-脱氧胞苷对卵巢癌细胞系CP70顺铂耐药性的逆转作用[J]. 生物磁学, 2012, 0(9): 1644-1647 |
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| 作者姓名： | 张琳琳 辛晓燕 张建芳 白露 高静 |
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| 作者单位： | 第四军医大学西京医院妇产科,陕西西安710032 |
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| 摘 要： | 目的：探究5-氮杂-2＇-脱氧胞苷体外逆转卵巢癌铂类耐药细胞系CP70对顺铂的耐药性,并探讨与SOCS-2表达的关系。方法：免疫细胞化学和Western blot方法检测使用5-氮杂-2＇-脱氧胞苷处理细胞前后细胞内SOCS-2表达水平。MTT法检测单独使用5-氮杂-2＇-脱氧胞苷或顺铂及两药联合使用对CP70细胞的抑制作用。结果：两药联合处理CP70细胞后顺铂耐药逆转倍数为1.34、1.63、2.34,联合处理组的细胞中SOCS-2表达明显升高。结论：5-氮杂-2＇-脱氧胞苷可以部分逆转CP70细胞对顺铂的耐药性,且此作用可能与增加细胞的SOCS-2表达有关。
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| 关 键 词： | 5-氮杂-2＇-脱氧胞苷 卵巢癌 顺铂 耐药性 |
5-Aza-CdR Resensitizes Cisplatin-resistant Human Ovarian Carcinoma Cells CP70 |
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| ZHANG Lin-lin,XIN Xiao-yan,ZHANG Jian-fang,BAI Lu,GAO Jing. 5-Aza-CdR Resensitizes Cisplatin-resistant Human Ovarian Carcinoma Cells CP70[J]. Biomagnetism, 2012, 0(9): 1644-1647 |
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| Authors: | ZHANG Lin-lin XIN Xiao-yan ZHANG Jian-fang BAI Lu GAO Jing |
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| Affiliation: | (Department of Obstetrics and Gynecology, Xijing Hospital, Fourth Military Medical University, Shanxi, Xi＇an, 710032, China ) |
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| Abstract: | Objective： To investigate the effect of 5-Aza-CdR on resensitizing cisplatin-resistant human ovarian carcinoma ceils CP70 and discuss the related mechanism. Methods： Immunohistochemical analysis and Western blot were used to detect the expression of SOCS-2 in different groups. MTT assay was used to assess the inhibitory effects of 5-Aza-CdR, cisplatin alone or a combination of both on CP70 cells. Results： The resensitizing fold of cisplatin combined with 5-Aza-CdR was 1.34, 1.63, 2.34. The expression of SOCS-2 had the most prominent decrease in CP70 cells in the combination group. Conclusion： 5-Aza-CdR can partlly resensitize cis- platin-resistant CP70 cells, which might be related to the down-regulation of SOCS-2. |
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| Keywords: | 5-Aza-CdR Ovarian carcinoma Cisplatin Drug resistance |
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