| M41型A群链球菌的Ⅰ型胶原样蛋白与人低密度脂蛋白的相互作用 |
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| 引用本文： | 梁纯伟,赵瑞东,高玉敏,韩润林.M41型A群链球菌的Ⅰ型胶原样蛋白与人低密度脂蛋白的相互作用[J].微生物学报,2010,50(7):903-908. |
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| 作者姓名： | 梁纯伟 赵瑞东 高玉敏 韩润林 |
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| 作者单位： | 1. 内蒙古农业大学生命科学学院,呼和浩特,010018;内蒙古农业大学血浆脂蛋白免疫学研究中心,呼和浩特,010018
2. 内蒙古农业大学兽医学院,呼和浩特,010018;内蒙古农业大学血浆脂蛋白免疫学研究中心,呼和浩特,010018 |
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| 基金项目： | 内蒙古农业大学引进人才科研启动金（K32615）；内蒙古高等学校科研项目（NJZY07004） |
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| 摘 要： | 【目的】检测M41型A群链球菌(GAS)ATCC12373中Ⅰ型胶原样蛋白(Scl1)与人低密度脂蛋白(LDL)的相互作用。【方法】克隆了M41型GAS ATCC12373的Ⅰ型胶原样蛋白(Scl1)及其V区(Scl1-V)基因,并表达、纯化重组蛋白rScl1(C176)和rScl1-V(C176V)。通过重组蛋白与人血浆的亲和色谱层析、Western blot及酶联免疫吸附试验(ELISA)检测C176、C176V与LDL的相互作用;通过GAS与LDL的ELISA试验和人血浆与GAS的共孵育试验,检测GAS与LDL的相互作用。【结果】结果证明C176和C176V可以与LDL特异性结合;表达Scl1的M41型GAS可以与LDL相结合。【结论】M41型GAS的Scl1可以与LDL特异性结合。
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| 关 键 词： | 关键词：A群链球菌 Ⅰ型胶原样蛋白 低密度脂蛋白 |
| 收稿时间： | 2009/12/9 0:00:00 |
| 修稿时间： | 2009/3/15 0:00:00 |
Scl1 of M41-type group A streptococcus binds low-density lipoprotein |
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| Chunwei Liang,Ruidong Zhao,Yuming Gao and Runlin Han.Scl1 of M41-type group A streptococcus binds low-density lipoprotein[J].Acta Microbiologica Sinica,2010,50(7):903-908. |
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| Authors: | Chunwei Liang Ruidong Zhao Yuming Gao and Runlin Han |
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| Institution: | College of Life Sciences Inner Mongolia Agricultural University, Hohhot 010018, China;College of Life Sciences Inner Mongolia Agricultural University, Hohhot 010018, China;Research Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Hohhot 010018, China;Research Center of Plasma Lipoprotein Immunology, Inner Mongolia Agricultural University, Hohhot 010018, China |
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| Abstract: | Abstract: Objective] To study the interaction of streptococcal collagen-like protein 1 (Scl1) of M41-type group A streptococcus (GAS) ATCC12373 with low-density lipoprotein (LDL). Methods] We cloned, expressed and purified the recombinant proteins rScl1 and its V region rScl1-V, designated as C176 and C176V, derived from Scl1.41 of M41-type GAS. The binding of rScl1 to LDL was detected with affinity chromatography-binding assay, Western blot analysis and enzyme-linked immunosorbent assay (ELISA), and whole cell binding assays were used to detect the interactions of whole GAS cells with LDL. Results] The results demonstrated that C176 and C176V could specifically bind purified LDL, and M41-type GAS cells expressing native Scl1 could bind LDL whereas M6-type GAS could not. Conclusion] The Scl1 of M41-type GAS specifically binds LDL. |
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| Keywords: | Keywords: group A streptococcus streptococcal collagen-like protein 1 low-density lipoprotein |
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