Plasmodium knowlesi: studies on invasion of rhesus erythrocytes by merozoites in the presence of protease inhibitors |
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| Authors: | T Hadley M Aikawa L H Miller |
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| Affiliation: | 1. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20205, U.S.A.;2. Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, U.S.A. |
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| Abstract: | The effect of protease inhibitors on invasion of rhesus erythrocytes by Plasmodium knowlesi merozoites was evaluated. Chymostatin, N-alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK), and L-1-tosylamide-2-phenylethylchloromethyl ketone (TPCK) inhibited invasion. Leupeptin, antipain, pepstatin, and phenylmethylsulfonyl fluoride (PMSF) had no effect. TLCK and TPCK inhibited attachment of merozoites to host erythrocytes. Chymostatin had no adverse effect on attachment, and in its presence junction formation between the merozoite and host erythrocyte occurred. Both chymostatin and leupeptin inhibited normal rupture of schizont-infected erythrocytes. It is suggested that proteolytic activity may be important both in the rupture of schizont-infected erythrocytes and in the invasion of erythrocytes by malaria parasites. |
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| Keywords: | Malaria Parasite Merozoite Invasion Attachment Junction formation Erythrocyte Chymostatin Leupeptin Antipain Pepstatin Protease Protease inhibitor TLCK TPCK PMSF phenylmethylsulfonyl fluoride |
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