A novel electrophoretic pattern of induction of rat liver microsomal membrane polypeptides by 2-acetylaminofluorene,nitrosamine and azo dye administration |
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| Authors: | R Cameron RN Sharma GD Sweeney E Farber RK Murray |
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| Institution: | 1. Dept. of Pathology, Univ. of Toronto, Toronto, Canada;2. Dept. of Biochemistry, Univ. of Toronto, Toronto, Canada;3. the Dept. of Medicine, McMaster University, Hamilton, Canada |
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| Abstract: | The electrophoretic patterns of the polypeptides of the microsomal membrane fraction of the livers of rats treated with various agents were compared. Administration of phenobarbital, or of benzoa]pyrene or 3-methylcholanthrene, resulted in specific increases of membrane polypeptides corresponding to cytochrome P-450 and cytochrome P-448 species respectively. Administration of 2-acetylaminofluorene, diethylnitrosamine, dimethylnitrosamine, N,N-dimethyl-4-aminoazobenzene or 3′-methyl-N,N-dimethyl-4-aminoazobenzene resulted in a marked increase of 2 other polypeptides, migrating just ahead of the phenobarbital-responsive cytochrome P-450 species. Preliminary evidence suggests that at least one of these 2 polypeptides may contain heme. The results indicate that administration of these N-containing carcinogens to rats results in a common electrophoretic pattern of induction of 2 specific microsomal membrane polypeptides. This pattern is different from those observed with classical inducers of the rat liver mixed-function oxidase system. |
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