Fluorometric determination of DNA in Chlamydomonas |
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| Authors: | Torleiv Lien Gjert Knutsen |
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| Institution: | 1. Institute for General Microbiology, University of Bergen, Norway;2. Botanical Laboratory, University of Bergen, Norway |
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| Abstract: | The diaminobenzoic acid dihydrochloride fluorescence method has been examined to determine optimal conditions for the assay of DNA in perchloric acid extracts and in pretreated Chlamydomonas cells on glass-fiber filters. Maximal fluorescence yield was obtained by allowing: (a) the perchloric acid extract to react with 4–6% diaminobenzoic acid dihydrochloride for 20–40 min at 60–80°C at about pH 3.0; (b) the pretreated cells on glass-fiber filters to react with a 20% solution of diaminobenzoic acid dihydrochloride for 40 min at 60°C. |
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