Metabolism of thyrotropin releasing factor in two clonal cell lines of nervous system origin |
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Authors: | Yvonne Grimm-Jørgensen SE Pfeiffer JF McKelvy |
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Institution: | Department of Biophysics, The Weizmann Institute of Science, Rehovoth, Israel;Laboratoire de Chimie Generale I, Université Libre de Bruxelles, 50 Avenue F. D. Roosevelt, Bruxelles, Belgique |
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Abstract: | Immunoreactive thyrotropin releasing factor (TRF) was detected in homogenates of two clonal cell lines, BN1010-1 and BN1010-3, derived from a rat central nervous system tumor. TRF was present in logarithmically-growing cells; daily medium changes with slightly acid culture medium (pH 6.8) greatly increased the TRF content of these cells. In contrast, TRF could not be detected in stationary phase cells. TRF peptidases were <1% as active in homogenates of BN1010 cells as those in homogenates of guinea pig brain or hypothalamus. It is expected that these cells will provide an excellent model system for the study of various aspects of TRF metabolism. |
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