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The formation and annealing of structural defects in lipid bilayer vesicles
Authors:Rüdiger Lawaczeck  Masatsune Kainosho  Sunney I. Chan
Abstract:It is shown that sonication of phospholipid-water dispersions below the crystalline → liquid crystalline phase transition temperature (Tc) produces bilayer vesicles with structural defects within the bilayer membrane, which permit rapid permeation of ions and catalyze vesicle-vesicle fusion. These structural defects are annihilated simply by annealing the vesicle suspension above Tc. The rate of annealing was found to be slow, of the order of an hour for T = 3 °C above Tc, but annealing is complete within 10 min for T = 10 °C above Tc. It is proposed that these structural defects are fault-dislocations in the bilayer structure, which arise from a population defect in the distribution of the lipid molecules between the outer and inner monolayers, when small bilayer fragments reassemble to form the small bilayer vesicles during the sonication procedure. Such a population defect can only be remedied by lipid transport via the inside ? outside flip-flop mechanism, which would account for the slow kinetics of annealing observed even at 3 °C above the phase transition.
Keywords:To whom requests for reprints should be sent.
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