1. Department of Physics, Jichi Medical School, 3311-1, Yakushiji, Minami-Kawachi, Kawachi-gun, Tochigi Prefecture, 329-04 Japan;2. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto Japan;3. Department of Biophysics, Faculty of Engineering Science, Osaka University, Osaka Japan
Abstract:
Kinetic studies of the isomerization reaction of horse heart ferricytochrome c between pH 8.5 and pH 12.1 have been carried out by using stopped-flow and rapid scanning stopped-flow techniques. Below pH 10, our results were in good agreement with the scheme proposed earlier (Davis, L. A., Schejter, A. and Hess, G. P. (1974) J. Biol. Chem. 249, 2624–2632). Above pH 10, another faster first-order process was observed, which suggested the existence of a transient species in the isomerization reaction between the species with and without a 695 nm band. The probable scheme of the isomerization reaction is considered to be
