Comparison of adhesive bond strength of different cell types in vitro |
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Authors: | M G Labant R D Fletcher |
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Institution: | (1) Department of Microbiology, School of Dental Medicine, University of Pittsburgh, 15261 Pittsburgh, Pennsyvania |
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Abstract: | Summary An established in vitro assay for quantitating cell-substratum adhesion has been utilized to measure the adhesiveness of 10
cell lines to a colloidal overlay. The procedure, a derivation of the William’s blister test for adhesives, involves growing
cells to confluency on a polystyrene surface and then overlaying the monolayers with a Bacto-agar substratum. The cell-agar
substratum systems are debonded and thera,adhesive bond strength, of the separation of the two interfaces calculated.
Thera’s were determined for the following cell types: SGL (gingival epithelial-like), L (transformed mouse fibroblasts), HeLa
(human carcinoma), MDCK (canine kidney epithelial), WI-38 (human embryonic lung), Flow 1000 (human embryonic skin—muscle),
Flow 4000 (human embryonic kidney), Flow 5000 (whole human embryo), BALB/c 3T3 (mouse fibroblasts) and SV40-transformed BALB/c 3T3 (simian virus 40-transformed mouse fibroblasts).
Transformed cells (L, HeLa and SV40-transformed BALB/c 3T3) proved to be quantitatively less adhesive (ra/cell) than either fibroblast or epithelial-like cell lines. Of the “normal” cells tested the kidney cells, human embryonic
fibroblast and canine epithelial cells, and the gingival epithelial-like cells demonstrated a weaker binding to the colloidal
overlay than the mouse fibroblasts (BALB/c 3T3), the human embryonic lung, the human embryonic skin-muscle, and the whole
human embryo fibroblast cell lines.
Concanavalin A increased the bonding strength of Flow 5000 cells after 24 hr incubation; however, the adhesiveness of the
treated BALB/c 3T3 cells remained similar to the unterested samples while thera of the treated SV40-transformed BALB/c 3T3 cells decreased.
This research was supported by National Institute of Dental Research Grant DE03983. |
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Keywords: | adhesion substrates cell culture |
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