Preliminary report of some hydrolytic-enzyme activities of honey bee semen |
| |
| Authors: | RALPH B. TURNER R. A.M. CHAVEZ-ARNOLD T. A. HOLGUIN |
| |
| Affiliation: | Entomology and Plant Pathology , New Mexico State University , Las Cruces , NM , 88003 , U.S.A. |
| |
| Abstract: | Semen, collected from active honey bee drones, was centrifuged to separate spermatozoa from seminal plasma. Enzymes were then extracted from spermatozoa with detergent buffer mixture at 37°C for 1 h. The following hydrolytic-enzyme activities were detected in seminal plasma and/or detergent extracts of sperm: protease (azocoll hydrolysis), hexosaminidase (N-acetyl-β-D-glucosaminidase), hyaluronidase, aryl sulfatase A and B. |
| |
| Keywords: | hydrolytic enzymes honey bee semen |
|
|
