田间栽培喜树不同品系枝叶喜树碱产量动态分析

采用高效液相色谱法和生物量法，以四川种源的普通喜树为对照，对生长季内喜树新品系海喜1号不同冠层枝叶喜树碱产量进行了测定。结果表明：由于海喜1号枝叶喜树碱含量、生物量均高于普通喜树，其产量更加显著地高于普通喜树；虽然海喜1号幼嫩的上层枝叶喜树碱含量高于中层和下层，但由于生物量偏低，产量明显低于中下层枝叶；虽然基于喜树碱含量较高（即优质为目标时），采收期应确定为6月份，但为了达到持续产量，在6月份可采收上层枝叶，7~10月以适度透光为方法连续采收中下部枝叶，11月份，即生长季末采收冠层下部全部1/3枝叶。

Dynamic Analysis on Camptothecin Yields in Branches and Leaves from Different Strains of Camptotheca acuminata in Field Condition

The yield of camptothecin (CPT) accumulated in two strains of Camptotheca acuminata Decne, i.e. Sichuan (SC) strain and Hisun-Ⅰ(HI) strain, were compared in filed condition using HPLC method. The results showed that the content of CPT, biomass accumulation and consequent CPT yields in H1 were significantly higher than those in SC strain. With regard to H1 strain, the content of CPT in the upper branches and leaves was much higher than those in the middle and bottom parts, while the yield was significantly reverse because of the low biomass. In June, the H1 strain contained more CPT in leaves than in other months. However, the biomass accumulation in June was comparatively lower. For the continuous yield purpose, the branches and leaves on the upper part was suggested to be harvested in June. However, the middle and bottom parts were harvested from July to October, and one third of which on the bottom part was harvested in November, the end of the growing season.