Birth of viable puppies derived from breeding cloned female dogs with a cloned male |
| |
Authors: | JE Park SG Hong JT Kang HJ Oh MK Kim MJ Kim HJ Kim DY Kim G Jang BC Lee |
| |
Institution: | aDepartment of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea;bDepartment of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungngam National University, Daejeon 305-764, Republic of Korea;cDepartment of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea |
| |
Abstract: | Since the establishment of production of viable cloned dogs by somatic cell nucleus transfer, great concern has been given to the reproductive abilities of these animals (Canis familiaris). Therefore, we investigated reproductive activity of cloned dogs by (1) performing sperm analysis using computer-assisted sperm analysis and early embryonic development, (2) assessing reproductive cycling by measuring serum progesterone (P4) levels and performing vaginal cytology, and (3) breeding cloned dogs using artificial insemination. Results showed that most parameters of sperm motility in a cloned male dog were within the reference range, and in vivo–matured oocytes from a noncloned female were successfully fertilized by spermatozoa from a cloned male dog and develop normally to the 8-cell stage. Three cloned female dogs displayed normal patterns of P4 levels and morphologic changes of the vaginal epithelium. Two cloned female dogs became pregnant using semen from a cloned male dog and successfully delivered 10 puppies by natural labor. In conclusion, these data demonstrated that both cloned male and female dogs are fertile, and their puppies are currently alive and healthy with normal growth patterns. |
| |
Keywords: | Artificial insemination Cloned dogs Pregnancy Reproductive ability Sperm motility |
本文献已被 ScienceDirect 等数据库收录! |
|