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Lactate from cultures of Lactobacillus casei recovered in a fluidized bed column using ion exchange resin
Authors:P R Córdoba  A L Ragout  F Siñeriz  N I Perotti
Institution:(1) PROIMI - UNT, Av. Belgrano y Pje. Caseros, 4000 SM Tucumán, Argentina
Abstract:Summary Lactic acid produced by continuous culture of L.casei in an upflow packed bed reactor, was recovered with Amberlite IRA 400 in a fluidized bed column. Bed expansions of 1.25 and 2.25 were applied. Reutilization did not alter the capability of net recovery of 0.048 ± 0.01 g lactic acid/g resin. When 2200 cm/h of ascensional velocity was used, (bed expansion of 2.25), the resin adsorbed 39.3% of the initial lactic acid and 63.5% was eluted. This resin supported the highest exchange capacity of 0.126 g lactic acid/g resin. Applying high flow rates, the process has potential industrial applications due to the short time employed.
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