Effects of pergolide mesylate on transduction efficiency of PEP-1-catalase protein |
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Authors: | Sohn Eun Jeong Kim Dae Won Kim Young Nam Kim So Mi Lim Soon Sung Kang Tae-Cheon Kwon Hyeok Yil Kim Duk-Soo Cho Sung-Woo Han Kyu Hyung Park Jinseu Eum Won Sik Hwang Hyun Sook Choi Soo Young |
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Institution: | aDepartment of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea;bDepartment of Food Science and Nutrition & RIC Center, Hallym University, Chunchon 200-702, Republic of Korea;cDepartment of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon 200-702, Republic of Korea;dDepartment of Physiology, College of Medicine, Hallym University, Chunchon 200-702, Republic of Korea;eDepartment of Anatomy, College of Medicine, Soonchunhyang University, Cheonan-Si 330-090, Republic of Korea;fDepartment of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea |
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Abstract: | The low transduction efficiency of various proteins is an obstacle to their therapeutic application. However, protein transduction domains (PTDs) are well-known for a highly effective tool for exogenous protein delivery to cells. We examined the effects of pergolide mesylate (PM) on the transduction of PEP-1-catalase into HaCaT human keratinocytes and mice skin and on the anti-inflammatory activity of PEP-1-catatase against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation using Western blot and histological analysis. PM enhanced the time- and dose-dependent transduction of PEP-1-catalase into HaCaT cells without affecting the cellular toxicity. In a mouse edema model, PEP-1-catalase inhibited the increased expressions of inflammatory mediators and cytokines such as cyclooxygenase-2, inducible nitric oxide synthase, interleukin-6 and -1β, and tumor necrosis factor-α induced by TPA. On the other hand, PM alone failed to exert any significant anti-inflammatory effects. However, the anti-inflammatory effect of co-treatment with PEP-1-catalase and PM was more potent than that of PEP-1-catalase alone. Our results indicate that PM may enhance the delivery of PTDs fusion therapeutic proteins to target cells and tissues and has potential to increase their therapeutic effects of such drugs against various diseases. |
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Keywords: | Pergolide mesylate Inflammation PEP-1-catalase Protein transduction |
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