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Species differences of macrophage very low-density-lipoprotein (VLDL) receptor protein expression |
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| Authors: | Takahashi Sadao Ito Takashi Zenimaru Yasuo Suzuki Jinya Miyamori Isamu Takahashi Masao Takahashi Masafumi Ishida Takafumi Ishida Tatsuro Hirata Ken-Ichi Yamamoto Tokuo T Iwasaki Tadao Hattori Hiroaki Shiomi Masashi |
| Institution: | aThird Department of Internal Medicine, University of Fukui, Faculty of Medical Science, Japan;bResearch and Education Program for Life Science, University of Fukui, Faculty of Medical Science, Japan;cInstitute for Experimental Animals, Kobe University Graduate School of Medicine, Japan;dDepartment of Cardiovascular Surgery, Hiratsuka Kyosai Hospital, Japan;eDivision of Bioimaging Sciences, Center for Molecular Medicine, Jichi Medical University, Japan;fDepartment of Cardiovascular Medicine, Hiroshima University Graduate School of Biomedical Sciences, Japan;gDivision of Cardiovascular Medicine, Kobe University Graduate School of Medicine, Japan;hCenter for Advanced Genome Research, Institute of Development, Aging, and Cancer, Tohoku University, Japan;iDepartment of Advanced Medical Technology and Development, BML, Inc., Japan;jSection of Animal Models for Cardiovascular Diseases, Kobe University Graduate School of Medicine, Japan |
| Abstract: | Triglyceride-rich lipoproteins (TGRLs) and low-density-lipoprotein (LDL) cholesterol are independent risk factors for coronary artery disease. We have previously proposed that the very low-density-lipoprotein (VLDL) receptor is one of the receptors required for foam cell formation by TGRLs in human macrophages. However, the VLDL receptor proteins have not been detected in atherosclerotic lesions of several animal models. Here we showed no VLDL receptor protein was detected in mouse macrophage cell lines (Raw264.7 and J774.2) or in mouse peritoneal macrophages in vitro. Furthermore, no VLDL receptor protein was detected in macrophages in atherosclerotic lesions of chow-fed apolipoprotein E-deficient or cholesterol-fed LDL receptor-deficient mice in vivo. In contrast, macrophage VLDL receptor protein was clearly detected in human macrophages in vitro and in atherosclerotic lesions in myocardial infarction-prone Watanabe-heritable hyperlipidemic (WHHLMI) rabbits in vivo. There are species differences in the localization of VLDL receptor protein in vitro and in vivo. Since VLDL receptor is expressed on macrophages in atheromatous plaques of both rabbit and human but not in mouse models, the mechanisms of atherogenesis and/or growth of atherosclerotic lesions in mouse models may be partly different from those of humans and rabbits. |
| Keywords: | Abbreviations: TGRLs triglyceride-rich lipoproteins VLDL very low-density lipoprotein LDL low-density-lipoprotein WHHLMI myocardial infarction-prone Watanabe-heritable hyperlipidemic apo apolipoprotein CADs coronary artery diseases |
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