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Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells |
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| Authors: | Toshikazu Tsuji Shigeko Kawai-Noma Chan-Gi Pack Hideki Terajima Junichiro Yajima Takayuki Nishizaka Masataka Kinjo Hideki Taguchi |
| Affiliation: | aDepartment of Biomolecular Engineering, Graduate School of Biosciences and Biotechnology, Tokyo Institute of Technology, B56, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan;bCellular Informatics Laboratory, RIKEN Advanced Science Institute, Wako-shi, Saitama 351-0198, Japan;cDepartment of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo 171-8588, Japan;dLaboratory of Molecular Cell Dynamics, Graduate School of Life Sciences, Hokkaido University, Sapporo 001-0021, Japan |
| Abstract: | Yeast is a model eukaryote with a variety of biological resources. Here we developed a method to track a quantum dot (QD)-conjugated protein in the budding yeast Saccharomyces cerevisiae. We chemically conjugated QDs with the yeast prion Sup35, incorporated them into yeast spheroplasts, and tracked the motions by conventional two-dimensional or three-dimensional tracking microscopy. The method paves the way toward the individual tracking of proteins of interest inside living yeast cells. |
| Keywords: | Quantum dots Single-particle tracking Yeast Yeast prion proteins |
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