<Emphasis Type="Italic">In vitro</Emphasis> regeneration and plant establishment of <Emphasis Type="Italic">Tylophora indica</Emphasis> (Burm. F.) Merrill: Petiole callus culture |
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Authors: | M?Faisal S?Singh Email author" target="_blank">M?AnisEmail author |
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Institution: | (1) Plant Tissue Culture Laboratory Department of Botany, Aligarh Muslim University, 202 002 Aligarh, India;(2) Plant Physiology Laboratory, Department of Botany, Aligarh Muslim University, 202 002 Aligarh, India |
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Abstract: | Summary A protocol has been developed for high-frequency shoot regeneration and plant establishment of Tylophora indica from petiole-derived callus. Optimal callus was developed from petiole explants on Murashige and Skoog basal medium supplemented
with 10μM2,4-dichlorophenoxyacetic acid +2,5μM thidiazuron (TDZ). Adventitious shoot induction was achieved from the surface of the callus after transferring onto shoot
induction medium. The highest rate (90%) of shoot multiplication was achieved on MS medium containing 2.5μM TDZ. Individual elongated shoots were rooted best on halfstrength MS medium containing 0.5μM indole-3-butyric acid (IBA). When the basal cut ends of the in vitro-regenerated shoots were dipped in 150μM IBA for 30 min followed by transplantation in plastic pots containing sterile vermiculite, a mean of 4.1 roots per shoot
developed. The in vitro-raised plantlets with well-developed shoot and roots were successfully established in earthen pots containing garden soil
and grown in a greenhouse with 100% survival. Four months after transfer to pots, the performance of in vitro-propagated plants of T. indica was evaluated on the basis of selected physiological parameters and compared with ex vitro plants of the same age. |
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Keywords: | callus culture ex-vitro rooting petiole explants photosynthesis Tylophora indica |
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