SSR与SRAP标记在玉米品种鉴定中的比较研究

利用SSR标记和SRAP标记对19个玉米品种及8份莱农15样品进行了分析,比较了2种标记的分辨能力及在亲缘关系和杂交种纯度鉴定中的表现.与SSR标记相比,SRAP标记用于玉米品种鉴定扩增住点数量更多,PIC值更高,具有更高的分辨率;在亲缘关系分析方面,SSR检测的遗传距离变幅更大,2种标记计算的遗传距离呈极显著正相关,分类结果基本一致;在杂交种纯度检测中,SRAP标记的期望位点在杂交种群体中检测率高于SSR标记相应位点,检测杂交种纯度结果更接近田问种植鉴定,因而准确度更高.SRAP在玉米品种鉴定中具有一定的优势,可作为SSR标记技术的有益补充,特别是在杂交种纯度鉴定中应用.

Comparison of SSR marker and SRAP Marker in maize varieties identification

SSR marker is usually the first choice of molecular markers in maize seed identification, but there stills exist some problems for SSR marker, especially in hybrids purity identification. It is still necessary to explore supplementary markers to facilitate varieties identification besides SSR marker. In this paper, SSR and SRAP were performed in 19 maize varieties and 9 samples of Lainong 15 about 1100 individuals. The discriminative power of the markers, genetic relationship and hybrids purity revealed by SSR and SRAP markers were compared and analyzed. Comparing to SSR markers, SRAP markers displayed more amplified alleles per primer-pair, higher PIC value, and higher discriminative power. For the 19 maize varieties, similar clustering results were achieved. Genetic Distance estimated based on SSR and SRAP data were significantly correlated with each other. Specific primers of SSR and SRAP with complementary pattern were used to detect hybrid purity of Lainong 15. The hybrid purity identified by SRAP marker was more vicinal to field test than that by SSR marker. SRAP markers could be applied in maize identification as a useful supplement of SSR, especially in hybrid purity identification.