Characterization of a novel flooding stress-responsive alcohol dehydrogenase expressed in soybean roots |
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Authors: | Setsuko Komatsu Deschamps Thibaut Susumu Hiraga Mikio Kato Mitsuru Chiba Akiko Hashiguchi Makoto Tougou Satoshi Shimamura Hiroshi Yasue |
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Institution: | (1) National Institute of Crop Science, Kannondai 2-1-18, Tsukuba 305-8518, Japan;(2) University Pierre et Marie Curie University, 75005 Paris, France;(3) Osaka Prefecture University, Osaka 599-8531, Japan;(4) National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan;(5) University of Tsukuba, Tsukuba 305-8577, Japan |
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Abstract: | Alcohol dehydrogenase (Adh) is the key enzyme in alcohol fermentation. We analyzed Adh expression in order to clarify the
role of Adh of soybeans (Glycine max) to flooding stress. Proteome analysis confirmed that expression of Adh is significantly upregulated in 4-day-old soybean
seedlings subjected to 2 days of flooding. Southern hybridization analysis and soybean genome database search revealed that
soybean has at least 6 Adh genes. The GmAdh2 gene that responded to flooding was isolated from soybean cultivar Enrei. Adh2 expression was markedly increased 6 h after flooding and decreased 24 h after floodwater drainage. In situ hybridization
and Western blot indicated that flooding strongly induces Adh2 expression in RNA and protein levels in the root apical meristem.
Osmotic, cold, or drought stress did not induce expression of Adh2. These results indicate that Adh2 is a flooding-response specific soybean gene expressed in root tissue. |
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