Human neutrophil lipocalin (HNL) is a specific granule constituent of the neutrophil granulocyte. Studies in bronchial and lung parenchymal tissue and peripheral blood cells |
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Authors: | L Sevéus Kawa Amin Christer G B Peterson Godfried M Roomans Per Venge |
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Institution: | (1) Pharmacia and Upjohn Diagnostics, S-75182 Uppsala, Sweden Tel. +46-18-163957; Fax +46-18-166465, SE;(2) Department of Human Anatomy, University of Uppsala, Box 571, S-75123 Uppsala, Sweden, SE;(3) Department of Clinical Chemistry, University of Uppsala, University Hospital, S-75185 Uppsala, Sweden, SE |
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Abstract: | The neutrophilic granulocyte is a cytotoxic and potentially tissue-injuring cell participating in the destructive processes
and symptoms seen in a variety of inflammatory diseases. Sensitive immunoassays have been introduced to measure the levels
of specific secretory proteins of various inflammatory cells in blood and other body fluids. The aim has been to develop highly
specific markers for each cell type. The results obtained by immunoassay have indicated that human neutrophil lipocalin (HNL)
is a protein unique to the neutrophil. The present study investigated the specificity of HNL as a neutrophil marker in peripheral
blood and lung tissue by using flow cytometry and immunocytochemistry. Flow cytometry and immunocytochemistry on peripheral
blood showed that monoclonal antibodies to HNL only react with neutrophils and not with other types of leukocytes. Immunocytochemistry
on plastic-embedded sections and on frozen sections of lung tissue showed that a cocktail of six monoclonal antibodies to
HNL specifically reacts with neutrophils and not with epithelial cells or macrophages. By immunoelectron microscopical studies
performed on healthy human neutrophils after low temperature embedding in Lowicryl K4M following aldehyde fixation and partial
dehydration, it could be shown that HNL colocalized with lactoferrin (a known marker for secondary or specific granules) and
that myeloperoxidase was localized in the primary or azurophil granules. The results confirm that HNL is a unique component
of the secondary granules of the neutrophil granulocyte.
Accepted: 8 January 1997 |
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