Primordial follicle assembly was regulated by notch signaling pathway in the mice |
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Authors: | Chun-Lei Chen Xia-Fei Fu Lin-Qing Wang Jun-Jie Wang Hua-Gang Ma Shun-Feng Cheng Zhu-Mei Hou Jin-Mei Ma Guo-Bo Quan Wei Shen Lan Li |
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Institution: | 1. Laboratory of Germ Cell Biology, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China 2. Center for Reproductive Biology, Weifang People’s Hospital, Weifang, 261041, China 3. Animal Husbandry Bureau of Qixia City, Qixia, 265300, China 4. Yunnan Animal Science and Veterinary Institution, Kunming, 650224, China
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Abstract: | Notch signaling pathway, a highly conserved cell signaling system, exists in most multicellular organisms. The objective of this study was to examine Notch signaling pathway in germ cell cyst breakdown and primordial follicle formation. The receptor and ligand genes of Notch pathway (Notch1, Notch2, Jagged1, Jagged2 and Hes1) were extremely down-regulated after newborn mouse ovaries were cultured then exposed to DAPT or L-685,458 in vitro (P < 0.01). Since DAPT or L-685,548 inhibits Notch signaling pathway, the expression of protein LHX8 and NOBOX was significantly reduced during the formation of the primordial follicles. Down-regulated mRNA expression of specific genes including Lhx8, Figla, Sohlh2 and Nobox, were also observed. The percentages of female germ cells in germ cell cysts and primordial follicles were counted after culture of newborn ovaries for 3 days in vitro. The result showed female germ cells in cysts was remarkably up-regulated while as the oocytes in primordial follicles was significantly down-regulated (P < 0.05). In conclusion, Notch signaling pathway may regulate the formation of primordial follicle in mice. |
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