产酸丙酸杆菌生长特性及5L罐发酵动力学研究

论文在摇瓶水平对产酸丙酸杆菌基本生长特性（温度、pH、摇床转速、接种量、种龄等）、碳源、氮源利用情况、产物抑制及5 L罐发酵动力学进行了研究。结果表明，该菌在32℃，初始pH 6．5，摇床转速150 r/min，接种24 h的种子液，接种量为5％条件下，产酸丙酸杆菌生长及产酸水平达最高值；该菌可利用碳源十分广泛，但对氮源要求比较高，只可利用有机氮源；在不同初始葡萄糖浓度下，产酸丙酸杆菌生长及产酸水平差异不大，无明显底物抑制现象；在2g/L的初始丙酸盐浓度下，该菌生长受到明显抑制；在5L发酵罐中，初始葡萄糖浓度为58．8 g/L，发酵72 h，葡萄糖消耗完全，丙酸终浓度达22．4 g/L，丙酸得率和产率分别达0．381 g/g和0．295 g/（L·h），丙酸占总酸比例达72．10％。

The Growth and 5 L Fermentor Kinetics Characteristics of Propionibacterium acidipropionici

The basic growth character including temperature,initial pH,speed of incubator,inoculum concentration, inoculum volume of Propionibacterium acidipropinici,the utilizable carbon and nitrogen source,the inhibition caused by the end product and the 5 L fermentor kinetics were studied in the wave bottle level.The result showed that it grew best at 32 ℃,and initial pH 6.5,with incubator 150 r/min and the inoculums volume 5% （24 h cultured broth mL /fresh medium mL）.The utilizable carbon sources were extensive but the only organic nitrogen sources was good for its growth;no evident substrate inhibition phenomenon was observed when cultured in different initial glucose concentrations;it could not grow well at the propionate concentration of 2 g/L;after 72 h of fermentation in 5 L fermentor,the glucose （58.8 g/L）was completely exhausted and the final propionic acid concentration reached 22.4 g/L,with propionic acid yield and productivity 0.382 g/g and 0.295 g/（L·h）respectively,propionic acid/total acids 72.10%.