Additional microsatellite markers for mouse genome mapping |
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| Authors: | Catherine M Hearne Marcia A McAleer Jennifer M Love Timothy J Aitman Richard J Cornall Soumitra Ghosh Andrew M Knight Jan-Bas Prins John A Todd |
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| Institution: | (1) Nuffield Department of Surgery, John Radcliffe Hospital, Headington, OX3 9DU Oxford, UK;(2) Transplantation Biology Section, Clinical Research Centre, Watford Road, HA1 3UJ Harrow, Middlesex, UK |
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| Abstract: | Mouse sequence information from the EMBL and GenBank databases, published sequences and genomic clones have been analyzed for simple repetitive elements or microsatellites. Each microsatellite has been amplified by the polymerase chain reaction (PCR) as a single locus marker. PCR primers were designed from unique sequence flanking each repeat. Size variation of PCR products less than 750 base pairs (bp) between mouse strains has been determined using ethidium bromide-stained acrylamide or agarose gels. A further 74 newly characterized microsatellites are presented in this paper, bringing to 185 the total we have analyzed. Of these, 157/185 (85%) have more than one allele, 143/178 (80%) vary in length between C57BL/6J and Mus spretus, and 82/168 (49%) vary between DBA/2J and C57BL/6J. Microsatellites provide informative single locus probes for linkage analysis in the construction of a genetic map of the mouse genome. |
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