Gram-Chromotrope: a New Technique that Enhances Detection of Microsporidial Spores in Clinical Samples |
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| Authors: | HERCULES MOURA JORGE L NUNES DA SILVA FERNANDO C SODRÉ PATRICIA BRASIL KRISTY WALLMO SUSANNE WAHLQUIST SARA WALLACE GIAN P CROPPO GOVINDA S VISVESVARA |
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| Institution: | Dept. Patologia &lab., Faculdade de Ciěncias Médicas, Univ. do Estado do Rio de Janeiro (UERJ);Hospital Evandro Chagas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil;Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Diseases Control, Atlanta, GA |
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| Abstract: | We have developed a new staining procedure that combines the traditional Gram staining for bacteria and the Weber's chromotrope staining method, the standard technique for the detection of microsporidia spores in clinical Specimens. This “Gram-chromotrope” staining technique enhances the staining characteristics of microsporidia spores and facilitates the easy detection and differentiation of spores from other microorganisms that are found in clinical specimens, especially stool samples. This new technique is fast, reliable, and simple to perform, and can be easily adapted for use in clinical laboratories. |
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| Keywords: | Microsporidia diagnosis staining methods |
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