Identification and characterization ofThermoplasma acidophilum 2-keto-3-deoxy-D-gluconate kinase: A new class of sugar kinases |
| |
Authors: | Jin Hwa Jung Sun Bok Lee |
| |
Institution: | (1) Department of Chemical Engineering and Division of Molecular Life and Sciences, Pohang University of Science and Technology, 790-784 Pohang, Korea |
| |
Abstract: | The thermoacidophilic archaeonThermoplasma acidophilum has long been known to utilized-glucosevia the non-phosphorylated Entner-Doudoroff (nED) pathway. We now report the identification of a gene encoding 2-keto-3-deoxy-d-gluconate (KDG) kinase. The discovery of this gene implies the presence of a glycolysis pathway, other than the nED pathway.
It was found that Ta0122 in theT. acidophilum genome corresponded to KDG kinase. This enzyme shares no similarity with known KDG kinases, and belongs to a novel class
of sugar kinases. Of the five sugars tested only KDG was utilized as a substrate. |
| |
Keywords: | d-gluconate kinase" target="_blank">2-keto-3-deoxy-d-gluconate kinase Thermoplasma acidophilum glycolysis non-phosphorylated Enter-Doudoroff pathway phosphoprotein Euryarchaeota |
本文献已被 SpringerLink 等数据库收录! |