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| 香荚兰细胞悬浮培养产生香兰素条件的研究 | |
| 引用本文： | 曹孟德,李家儒,王君健. 香荚兰细胞悬浮培养产生香兰素条件的研究[J]. 生物技术, 1999, 9(1): 8-10,32 |
| 作者姓名： | 曹孟德 李家儒 王君健 |
| 作者单位： | 1. 河南医科大学博士后流动站,郑州,450052 2. 中国科学院武汉水生生物研究所,武汉,430072 3. 华中理工大学生物工程系,武汉,430074 |
| 摘 要： | 该文报道了碳源、氮源及吸附剂对香荚兰细胞悬浮培养产生香兰素的影响，结果表明，蔗糖比葡萄糖及果糖更适合作香荚兰细胞生长及产生香兰素的碳源，最佳蔗糖浓度为５％；当培养基中仅含ＫＮＯ３，则有利于细胞的生长和香兰素的形成，培养液中去掉ＫＮＯ３仅含ＮＨ４ＮＯ３时，细胞生长和香兰素形成均被抑制；培养基添加吸附剂后，香美兰细胞产生的香兰素含量明显增加，活性炭的效果优于ＸＡＤ-２，而且活性炭用量增加，香兰素的产量亦增加。 |
| 关 键 词： | 香荚兰 香兰素 悬浮培养 |
STUDY ON THE CONDITIONS OF ANILLIN PRODUCATION BY BATCH CELLSUSPENSION CULTURE OF VANILLA PLANIFOLIA |
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| Cao Mengde, Li Jiaru, Wang Junjian. STUDY ON THE CONDITIONS OF ANILLIN PRODUCATION BY BATCH CELLSUSPENSION CULTURE OF VANILLA PLANIFOLIA[J]. Biotechnology, 1999, 9(1): 8-10,32 | |
| Authors: | Cao Mengde Li Jiaru Wang Junjian |
| Affiliation: | Cao Mengde1; Li Jiaru2; Wang Junjian3(1. Department of Basic Science;;Henan Medical U niversity.Zheng zhou;;450052)(2. Institute of Hydrobiology.The Chinese Academy of Science;;Wuhan;;430074)(3. Bioengineering D epartment.Huazhong U Niversity of Science |
| Abstract: | This article reported the effects of the kinds and concentration of carbon sources.nitrogen sources and absorbents on the cell growth and producation of vaniliin of Yanillaplanifonia cells in batch suspension culture. It was shown that sucrose was the better carbonsource than glucose and fructose foe the cell growth and vanillin formation: and when itsconcentrion in MS medium was of 5 percentage. the biosynthesis of vanillin had thelargestnumerical value: nitrate -- nitrogen improved cell growt}l and vanillin producation. butammonia-nitrogen inhibited vanillin formation;;;; the usage of absorbents (e. g. XAD - 2.activated charcoal) had a marked increasing effect on the accumulation of vanillin. |
| Keywords: | Vallisa planifonia vanillin suspension culture |
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