Inheritance of mitochondrial DNA in lentil (Lens culinaris Medik.) |
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| Authors: | O P Rajora J D Mahon |
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| Institution: | (1) Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9 Saskatoon, Saskatchewan, Canada;(2) Present address: Department of Renewable Resources, University of Alberta, T6G 2H1 Edmonton, Alberta, Canada |
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| Abstract: | Restriction fragment analysis was used to examine the inheritance of lentil mitochondrial DNA (mtDNA) in F1 and F5 progeny from intrasubspecific (Lens culinaris ssp. culinaris) crosses and in F1 progeny from intersubspecific (Lens culinaris ssp. orientalis x L. culinaris ssp. culinaris) crosses. Southern blots of digested parental and progeny DNA were hybridized to heterologous maize mtDNA probes specific to coxI and atp6 genes. Two restriction fragment polymorphisms separated L.c. ssp. culinaris  Laird and Eston from L.c. ssp. culinaris ILL5588, and one restriction fragment polymorphism distinguished L.c. ssp. culinaris Laird and Eston from L.c. ssp. orientalis LO4. Twelve of 13 f1 progeny and all F5 progeny from the intrasubspecific crosses, and all F1 progeny from intersubspecific crosses had only maternal mtDNA restriction fragments. One f1 plant from an Eston x ILL5588 cross inherited mtDNA fragments from both parents. Nuclear DNA inheritance was biparental in all F1 progeny.NRCC No. 38451 |
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| Keywords: | Lens culinaris Mitochondrial DNA Maternal inheritance Biparental mitochondrial inheritance Restriction fragment length polymorphism |
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