| 若干蝙蝠葛碱衍生物对人红细胞膜Ca~(2+)-Mg~(2+)-ATPase活力的影响 |
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| 引用本文： | 李志强,贡岳松,胡卓逸.若干蝙蝠葛碱衍生物对人红细胞膜Ca~(2+)-Mg~(2+)-ATPase活力的影响[J].中国生物化学与分子生物学报,1993,9(6):675-679. |
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| 作者姓名： | 李志强 贡岳松 胡卓逸 |
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| 作者单位： | 中国药科大学生物化学研究室，中国药科大学生物化学研究室，中国药科大学生物化学研究室 南京 210009 河南，开封，开封医学专科学校生物化学教研室475001，南京 210009，南京 210009 |
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| 摘 要： | 本文测定了数种蝙蝠葛碱衍生物对钙调素(CaM)激活的人红细胞膜Ca~(2+)-Mg~(2+)-ATPase活力的影响。结果表明,这些化合物对该酶都有不同程度的抑制作用,其机制表现为竞争性抑制,过量的CaM能完全逆转这些化合物所引起的抑制。当Ca~(2+)-Mg~(2+)-ATPase被胰蛋白酶(trypsin)限制性酶解完全活化后,其活力不再受CaM激活,但仍被这些化合物所抑制。
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| 关 键 词： | 红细胞膜Ca~(2+)-Mg~(2+)-ATPase 钙调素 蝙蝠葛碱衍生物 限制性酶解 |
| 收稿时间： | 1993-12-20 |
Effects of Dauricine Derivatives on Ca~(2+)-Mg~(2+)-ATPase Activity of Human Erythrocyte Membranes |
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| Li,Zhi-qiang Gong,Yue-song Hu,Zhuo-yi.Effects of Dauricine Derivatives on Ca~(2+)-Mg~(2+)-ATPase Activity of Human Erythrocyte Membranes[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(6):675-679. |
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| Authors: | Li Zhi-qiang Gong Yue-song Hu Zhuo-yi |
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| Institution: | (Division of Biochemistry, China Pharmaceutical University, Nanjing,210009 |
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| Abstract: | The effects of dauricine derivatives on Ca~(2+)-Mg~(2+)-ATPase activity of human erythrocyte membranes were detected. The results showed that these compounds inhibited CaM-activated Ca~(2+)-Mg~(2+)-ATPase activity according to a competitive mechanism. Increasing concentration of CaM completely reversed the inhibition caused by these compounds. Ca~(2+)-Mg~(2+)-ATPase could be activated by limited trypsinization in the absence of CaM. The trypsin-stimulated Ca~(2+)-Mg~(2+)-ATPase could not be further activated by the addition of CaM, but could be inhibited by these compounds. |
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| Keywords: | Calmodulin Erythrocyte membranes Ca~(2+)-Mg~(2+)-ATPase Dauricine deri-vative Limited trypsinization |
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