Respiratory Syncytial Virus Isolation by Combined Continuous Flow-Isopycnic Banding Centrifugation |
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| Authors: | G B Cline Helen Coates N G Anderson R M Chanock and W W Harris |
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| Institution: | Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830;Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014;Technical Division, Oak Ridge Gaseous Diffusion Plant, Oak Ridge, Tennessee 37830 |
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| Abstract: | A new zonal centrifuge rotor (B-IX) which combines continuous sample flow centrifugation with isopycnic banding has been used to isolate and concentrate respiratory syncytial virus from liter volumes of culture fluid. This isolation technique utilizes a sucrose density gradient to trap and isopycnically band the virus particles, and permits recovery of the particles from the rotor in an unaggregated condition. |
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