| 5-羟色胺在豚鼠胰腺分布的免疫组织化学研究 |
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| 引用本文： | 张远强,苏慧慈,黄荫乔,孙岚.5-羟色胺在豚鼠胰腺分布的免疫组织化学研究[J].中国组织化学与细胞化学杂志,1992(2). |
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| 作者姓名： | 张远强 苏慧慈 黄荫乔 孙岚 |
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| 作者单位： | 第四军医大学组织学与胚胎学教研室，第四军医大学组织学与胚胎学教研室，第四军医大学组织学与胚胎学教研室，第四军医大学组织学与胚胎学教研室 西安 710032，西安 710032，西安 710032，西安 710032 |
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| 摘 要： | 本研究用ABC免疫染色法,结合葡萄糖氧化酶-DAB-硫酸镍铵(Glucose oxidase-DAB-Nickle,GDN)显色技术,在Bouin液固定的常规石蜡切片上,研究了5-羟色胺(5-hydroxytryptamin,5-HT)在豚鼠胰腺内的定位和分布,并用相邻切片免疫双标记,观察了它与胰岛素的共存关系,结果发现,在豚鼠胰腺内,外分泌部均有5-HT免疫反应细胞分布。在胰腺内分泌部(胰岛)5-HT免疫反应细胞分布均匀,大部分胰岛细胞呈阳性5-HT样免疫反应,用相邻薄切片免疫双标记技术证明,胰岛内的5-HT免疫反应细胞主要是B细胞。在胰腺外分泌部,5-HT免疫反应细胞呈单个分散或聚集分布,主要位于腺泡和导管等处,偶见于结缔组织间隔中。本文对研究5-HT在胰腺的生理作用及其机制提供了形态学依据。
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| 关 键 词： | 5-羟色胺 胰腺 免疫组织化学 豚鼠 |
IMMUNOHISTOCHEMICAL LOCALIZATION OF 5-HYDROXYTRYPTAMIN (5-HT) IN GUINEA PIG PANCREAS |
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| Zhang Yuanqiang,Su Huici,Huang Yinqiao and Sun Lan.IMMUNOHISTOCHEMICAL LOCALIZATION OF 5-HYDROXYTRYPTAMIN (5-HT) IN GUINEA PIG PANCREAS[J].Chinese Journal of Histochemistry and Cytochemistry,1992(2). |
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| Authors: | Zhang Yuanqiang Su Huici Huang Yinqiao and Sun Lan |
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| Abstract: | The localization and distribution of 5-HT in guinea-pig pancreas were investigated with ABC immunostaining method combined with the glucose oxidase-DAB-Nickle developing technique on paraffin sections. In this study, It was demonstrated that 5-HT immunoreactive cells were present both in the endocrine (islets)and exocrine pancreas of the guinea pig. The 5-HT immunoreactive cells in the pancreatic islets were identical in distribution and multiformed in shape. The majority of islets cells showed positive 5-HT immunostaining. The coexistence of 5-HT and insulin in the pancreatic islets was also proved with immunostaining of adjacent thin sections. 5-HT immunoreactive cells were also found in the epithelium of the ducts and acini of the exocrine pancreas. The significance of these results needs farther study. |
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| Keywords: | 5-Hydroxytryptamin Pancreas Immunohistochemistry Gguinea-pig |
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