马铃薯试管诱导结薯方法的改进

本研究对马铃薯试管诱导结薯的方法作了一些改进。用带有4—5个节的马铃薯无病毒茎段,在加有生长因子的MS液体培养基中作浅层静置培养,2—3周后,可长成3~2—4~2个枝条。此时,将诱导结薯的液体培养基加入同一容器内,培养1—3周后,小植株的多数茎节上开始出现气生小块茎。再经过3—4周的培养,小块茎成熟并具有发芽能力,可以收获作为无病毒超级原种加以应用。这种改进了的方法可以大大缩短无病毒小薯的生产周期,节约成本、提高产量。讨论了激素等培养条件对小块茎形成的影响和将试管诱导结薯技术应用于马铃薯无病毒种薯生产的前景。

IMPROVEMENTS IN INDUCING IN VITRO TUBERS OF POTATO

t A scries of experiments were conducted to improve the induction of in vitro tuberization of potato. The results showed that a length of shoot with 3 to 4 nodes cut off from in vitro potato plantlet could be proliferated to 3~2 to 4~2 shoots in 2 to 3 weeks when it was cultured in static shallow liquid Murashigc-Skoog medium which had been added with some growth agents. Then these shoots would initiate tuberization in 1 to 3 weeks just after replacing the existing medium by the tuber inducing medium into the same container. In another 3 to 4 weeks, these tubcrlets matured and were capable of sprouting. They could be harvested as virusfree prebasie secdpotatoes for cither further propagation or direct utilization. By this improved method, the process of the propagation of virusfree tubcrlets. was much accelerated. Also the cost of material and labor was decreased and the quantity of the virusfree prebasic seedpotatoes was greatly increased. The effects of hormones and the application of the improved management to the production of the virusfree secdpotatoes, are discussed in this paper.