Spatial distribution of three phytochromes in dark- and light-grown Avena sativa L. |
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Authors: | Yu-Chie Wang Marie-Michèle Cordonnier-Pratt Lee H Pratt |
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Institution: | (1) Botany Department, University of Georgia, 30602 Athens, GA, USA;(2) Present address: Institute of Molecular Biology, Academia Sinica, 11529 Nankang, Taipei, Taiwan, ROC |
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Abstract: | We have addressed two issues regarding the spatial distribution of three phytochromes in 3-d-old oat (Avena sativa L.) seedlings. Three monoclonal antibodies, GO-4, GO-7 and Oat-22, were used as probes. Each antibody detects only one of
the phytochromes. The first issue is whether any of the phytochromes might be membrane-bound. To address this issue the abundance
of each phytochrome in extracts prepared with either a detergent-free or a detergent-containing buffer was compared by immunoblot
assay. The detergent-free buffer was formulated to extract only soluble protein, while the detergent-containing buffer was
intended to extract both soluble and membrane proteins. None of the data indicate that any of these three phytochromes is
membrane-bound in either a dark- or a light-grown seedling. The second issue is whether these three phytochromes are distributed
differentially in 3-d-old dark- and light-grown seedlings. When seedlings were dissected into shoots, scutellums, and roots,
all three phytochromes were detected in all three fractions from both dark- and light-grown seedlings. Each of the three phytochromes
was most abundant in the shoot and least abundant in the root, except that in light-grown seedlings type I, etiolated-tissue
phytochrome was more abundant in the root than in either the shoot or the scutellum. When the equivalent fractions dissected
from different seedlings were compared, those dissected from dark-grown seedlings contained a higher quantity of each of the
three phytochromes than did those dissected from light-grown seedlings, except that green-tissue, type II phytochromes did
not differ significantly in the roots. At this level of resolution, no evidence was obtained to indicate a substantive difference
among the three phytochromes in their spatial distribution.
We thank Drs. Elizabeth Williams and Tammy Sage (Botany Department, University of Georgia, USA) for generously permitting
us to use their image-analysis system. This research was supported by USDA NRICGP grant 91-37100-6490. |
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Keywords: | Avena Phytochrome (spatial distribution) Spatial distribution (phytochrome) |
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