| Xeno-free derivation and culture of human embryonic stem cells： current status, problems and challenges |
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| 作者姓名： | Lei T Jacob S Ajil-Zaraa I Dubuisson JB Irion O Jaconi M Feki A |
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| 作者单位： | [1]Laboratory of Stem Cell Research, Department of Obstetrics and Gynecology, Geneva University Hospitals, Geneva, Switzerland [2]Department of Obstetrics and Gynecology, Geneva University Hospitals, Geneva, Switzerland [3]Department of Pathology and Immunology, Faculty of Medicine, Geneva University, Geneva, Switzerland |
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| 基金项目： | Acknowledgments We gratefully acknowledge the support of the Swiss National Foundation for Scientific Research. |
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| 摘 要： | Human embryonic stem cells （hESC） not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications.
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| 关 键 词： | 胚胎干细胞 细胞质量 细胞分化 实验研究 |
Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges |
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| Lei T,Jacob S,Ajil-Zaraa I,Dubuisson JB,Irion O,Jaconi M,Feki A.Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges[J].Cell Research,2007,17(8):682-688. |
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| Authors: | Lei Ting Jacob Sandrine Ajil-Zaraa Imen Dubuisson Jean-Bernard Irion Olivier Jaconi Marisa Feki Anis |
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| Institution: | Laboratory of Stem Cell Research, Department of Obstetrics and Gynecology, Geneva University Hospitals, Geneva, Switzerland. |
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| Abstract: | Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications. |
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| Keywords: | embryonic stem cells inner cell mass derivation feeder cells xeno-free differentiation |
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