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Identification of newly isolated Babesia parasites from cattle in Korea by using the Bo-RBC-SCID mice
Authors:Cho Shin-Hyeong  Kim Tong-Soo  Lee Hyeong-Woo  Tsuji Masayoshi  Ishihara Chiaki  Kim Jong-Taek  Wee Sung-Hwan  Lee Chung-Gil
Institution:Department of Medical Zoology, National Institute of Health, Seoul, 122-701 Korea.
Abstract:Attempts were made to isolate and identify Korean bovine Babesia parasite. Blood samples were collected from Holstein cows in Korea, and Babesia parasites were propagated in SCID mice with circulating bovine red blood cells for isolation. The isolate was then antigenically and genotypically compared with several Japanese isolates. The Korean parasite was found to be nearly identical to the Oshima strain isolated from Japanese cattle, which was recently designated as Babesia ovata oshimensis n. var. Haemaphysalis longicornis was the most probable tick species that transmitted the parasite.
Keywords:babesiosis  SCID mice  western blotting  polymerase chain reaction  phylogeny  Haemaphysalis longicornis
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