| 香蕉茎尖超低温保存过程中的细胞超微结构观察(简报) |
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| 引用本文： | 吴黎明,曾继吾,彭抒昂,易干军,牛王翠.香蕉茎尖超低温保存过程中的细胞超微结构观察(简报)[J].分子细胞生物学报,2006,39(6):563-567. |
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| 作者姓名： | 吴黎明 曾继吾 彭抒昂 易干军 牛王翠 |
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| 作者单位： | 广东省农业科学院果树研究所，广东省农业科学院果树研究所，华中农业大学园艺林学学院，广东省农业科学院果树研究所，广东省农业科学院果树研究所 广州 510640 华中农业大学园艺林学学院，武汉 430070 湖北省农业科学院果树茶叶研究所，武汉 430209，广州 510640，武汉 430070，广州 510640，广州 510640 华中农业大学园艺林学学院，武汉 430070 |
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| 基金项目： | 广东自然科学基金项目资助(05103391)。 |
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| 摘 要： | 超低温保存(Cryopreservation)通常称为液氮保存或LN(-196℃)保存,是目前植物种质资源长期稳定保存的理想方法,已经成功应用于多种植物种质资源保存。玻璃化法(Vitrification)超低温保存植物种质资源始于20世纪80年代末,Uagami等首次
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| 关 键 词： | 香蕉 超低温保存 玻璃化法 超微结构 |
| 收稿时间： | 2005-10-31 |
| 修稿时间： | 2006-09-10 |
ULTRASTRUCTURAL OBSERVATION OF BANANA SHOOT-TIP CELL DURING CRYOPRESERVATION BY VITRIFICATION |
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| WU Li Ming,ZENG Ji Wu,PENG Shu Ang,YI Gan Jun,NIU Wang Cui.ULTRASTRUCTURAL OBSERVATION OF BANANA SHOOT-TIP CELL DURING CRYOPRESERVATION BY VITRIFICATION[J].Journal of Molecular Cell Biology,2006,39(6):563-567. |
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| Authors: | WU Li Ming ZENG Ji Wu PENG Shu Ang YI Gan Jun NIU Wang Cui |
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| Institution: | 1 Fruit Research Institute,Guangdong Academy of Agricultural Science, Guangdong 510640; 2 College of Horticulture ,Huazhong Agricultural University, Wuhan 430070; 3 Institute of Fruit Tea,Hubei Academy of Agricltural Sciencs,Wuhan 430070 |
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| Abstract: | Excised shoot-tips produced from banana plants belonging to cv.Guangdong No.1 (ABB group) were eryopreserved suecessfully by vitrification using the PVS_2 solution.Uhrastruc- tural of banana shoot-tips eells was also observed by using eleetron micryoscopy (TEM).The re- sults showed that the plasmolysis became more and more severe during the course of dehydration. Cells were mainly damaged during the freezing and thawing process.Most cell protoplasts con- densed,and cell organelles,cell membranes and nucleus envelopes were lethally injured after cry- opreservation.But only a few cells located in the meristematic dome arose reversible process al- though their structures were varied.They could survive and regenerate plantlets after freezing con- servation. |
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| Keywords: | Banana Cryopreservation Vitrification Uitrastructure |
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