TaDEP1基因在普通六倍体小麦及其供体种中的保守与分化

根据已知小麦正源基因TaDEP1 cDNA序列设计引物,成功克隆了小麦TaDEP1基因组序列,发现该基因包含5个外显子,4个内含子.通过比较该基因在六倍体普通小麦A、B、D基因组中的差异,筛选出可以区分A、B、D基因组的分子标记Ta956.以中国春缺体-四体系为材料,利用该标记将TaDEP1基因定位于小麦5A、5B和5...

Conservation and divergence of TaDEP1 genes in common wheat (Triticum aestivum L.) and its donor species

The rice DEP1 (DENSE AND ERECT PANICLE1) gene is an important regulator that can enhance meristematic activity, reduce internode distance, increase per spike grain number, and eventually increase the rice yield. We report here the cloning of the genomic sequence of the wheat orthologous gene TaDEP1 which contains five exons and 4 introns. Genomic sequence comparison revealed a region that can be used as molecular marker (Ta956) to distinguish homoeoalleles of hexaploid wheat. Ta956 was used to map TaDEP1 to wheat chromosomes 5A, 5B, and 5D by using Chinese Spring nulli-tetrasomic lines. Sequences corresponding to Ta956 were then isolated and sequenced from hexaploid wheat, its putative donor species and tetraploid wheat. Sequence comparison showed that most variations were present at introns, particularly between the B and D homoeoalleles. Domain analysis showed that proteins from the D genome of hexaploid wheat was more different to those on the A and D genomes, suggesting functional divergence among TaDEP1 proteins from the A, B, and D genomes. The potential of using Ta956 as a marker for variety identification is discussed.