Effects of antimycotics on the biosynthesis of cellular macromolecules inAspergillus niger protoplasts |
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| Authors: | Kibong Oh Prof. Hideaki Matsuoka Tohru Teraoka Osao Sumita Kosuke Takatori Hiroshi Kurata |
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| Affiliation: | (1) Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16, Nakamachi, Koganei, 184 Tokyo, Japan;(2) Department of Plant Protection, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan;(3) Research and Development Division, Bio-Giken Inc., Tokyo, Japan;(4) Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan;(5) The Tokyo Kembikyoin Foundation, Tokyo, Japan |
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| Abstract: | The effects of nine antimycotics on the biosynthesis of cellular macromolecules were analyzed using the regenerating system of protoplats ofAspergillus niger. The incorporation of several specific radioactive precursors into major cellular components were measured in the presence or in the absence of respective agents. Miconazole, ketoconazole, and tolnaftate inhibited the lipid synthesis. 5-Fluorocytosine strongly inhibited the DNA and protein syntheses. Griseofulvin, however, specifically inhibited the synthesis of cell wall polysaccharides, i.e. chitin and glucan. Other agents showed non-specific inhibition effects. The significance of morphological change of hypha as an indicator of antimycotic action and its feasibility as a screening tool for novel antimycotic compounds are discussed.Abbreviations GRP germination of regenerating protoplasts - TCA trichloroacetic acid |
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| Keywords: | Antimycotics Biosynthesis of macromolecules Protoplasts Radioactive precursors |
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