Rituximab sensitizes a Burkitt lymphoma cell line to cell killing by X-irradiation |
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Authors: | Min Fengling Liu Fenju Wen Wanxin Zhai Lijia Tong Jiandong Wang Zu Yuan Xin Gao Qingxiang |
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Institution: | (1) School of Radiation Medicine and Public Health, Soochow University, 215006 Suzhou, China;(2) Department of Hematology, Yangzhou First People’s Hospital, 225001 Yangzhou, China;(3) School of Life Sciences, Lanzhou University, 730000 Lanzhou, China; |
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Abstract: | Clinical trials with rituximab in combination with chemotherapeutic regimens have shown promising results. Data on the effects
of rituximab treatment in combination with irradiation are, however, limited and inconsistent. This study aims to investigate
the effects of rituximab (R) on cell death induced by X-irradiation in Raji lymphoma cells and to evaluate its mechanisms.
We found the cell growth inhibition by irradiation was enhanced by additional rituximab exposure both in cells precultured
with rituximab followed by irradiation (R + irradiation) or in cells treated in the reverse sequence (irradiation + R). R + irradiation
combination treatment induced more apoptotic cells than irradiation and irradiation + R treatment as early as 12 h after treatment.
At 24 h, both combination treatments, R + irradiation and irradiation + R, showed apoptotic cells, which were significantly
different from irradiation alone. G2/M cell cycle arrest was observed after irradiation alone and the combination treatment.
The combination treatment revealed an elevation in reactive oxygen species (ROS) generation in a radiation dose-dependent
manner. In addition, rituximab enhanced the cell growth inhibition and apoptotic cell death induced by the oxidative agent,
H2O2. We propose that rituximab mediates a significant in vitro radiosensitizing effect and induces cell cycle changes and apoptosis
in Raji cells. ROS probably play an important role in these events. |
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