Phosphorylation of neurofilament proteins by endogenous calcium/calmodulin-dependent protein kinase |
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| Authors: | M L Vallano T M Buckholz R J DeLorenzo |
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| Affiliation: | 1. Department of Biomedical Sciences, University of Lausanne, Rue du Bugnon 7a, CH-1005 Lausanne, Switzerland;2. Centre for Organismal Studies (COS), University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany |
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| Abstract: | A protein fraction containing neurofilaments was prepared from rat brain cytosol by differential centrifugation and gel filtration chromatography. These preparations were enriched for a calcium/calmodulin-dependent kinase activity that phosphorylated endogenous neurofilament proteins. The enzyme incorporated approximately 1 mol PO4/mol of each neurofilament triplet polypeptide. These data suggest that a calmodulin-dependent kinase may mediate some of the effects of calcium on cytoskeletal function by phosphorylation of neurofilament proteins. |
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