| 核酸定量检测方法研究进展 |
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| 引用本文： | 谢浩,胡志迪,赵明,甘绍举,丁先锋. 核酸定量检测方法研究进展[J]. 生命的化学, 2014, 0(6): 737-743 |
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| 作者姓名： | 谢浩 胡志迪 赵明 甘绍举 丁先锋 |
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| 作者单位： | 浙江理工大学生命科学学院,杭州310018 |
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| 摘 要： | 核酸相关的研究和应用广泛存在于各个学科领域,与之相关的核酸定量检测技术越来越受到研究人员的重视。本文对目前实验室广泛采用的和近几年进展迅速的核酸定量方法(包括紫外分光光度法、荧光染料法、实时荧光定量PCR法、数字PCR法等)进行介绍,着重阐述其检测原理和优缺点,为研究人员今后进行相关研究提供参考。
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| 关 键 词： | 核酸 定量 检测方法 |
Research progress of the methods for nucleic acid quantitive detection |
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| XIE Hao,HU Zhidi,ZHAO Ming,GAN Shaoju,DING Xianfeng. Research progress of the methods for nucleic acid quantitive detection[J]. Chemistry of Life, 2014, 0(6): 737-743 |
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| Authors: | XIE Hao HU Zhidi ZHAO Ming GAN Shaoju DING Xianfeng |
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| Affiliation: | (College of Life Science, Zhejiang Sci-Tech University, Zhejiang 310018, China) |
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| Abstract: | Nucleic acid-related research and applications is widespread in various disciplines. Researchers pay increasing attention to the nucleic acid quantitative detection technologies. Focusing on the detection prin- ciple and the merits and demerits, we will introduce some technologies （including ultraviolet spectrophotometry, fluorescent dye method, RQ-PCR, dPCR, etc.） that are widely used in laboratories and the rapid research progress of those technologies in recent years, so as to provide a reference for the future researches. |
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| Keywords: | nucleic acid quantitation detection method |
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