Physical and functional mapping of rat-liver mitochondrial DNA期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Physical and functional mapping of rat-liver mitochondrial DNA

Authors:	T. B. Kazakova S. G. Babich M. P. Mel'nikova N. V. Tzimbalenko E. A. Braga V. V. Nosikov

Affiliation:	(1) Institute of Experimental Medicine of the USSR Academy of Medical Sciences, Leningrad, USSR;(2) Institute of Molecular Biology of the USSR Academy of Sciences, Moscow, USSR

Abstract:	Summary Rat-liver mitochondrial DNA (mtDNA) contains 2 cleavage sites of the restriction endonuclease XbaI. The molecular sizes of restriction fragments are 6.6×10⁶ and 3.7×10⁶ D. The results of partial cleavage of mtDNA with EcoRI allow the fragment F (0.32×10⁶ D) to be localized in the sequence ABCEGFHDA. The functional map of mtDNA is constructed for two genes of the ATP-ase mRNAs from rat-liver mitochondria. Molecular hybridization shows that the ATPase genes are located in fragment B and in the GEHD area of mtDNA EcoRI cleavage.

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