Four receptor‐like cytoplasmic kinases regulate development and immunity in rice |
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| Authors: | Xiaogang Zhou Jing Wang Chunfang Peng Xiaobo Zhu Junjie Yin Weitao Li Min He Jichun Wang Mawsheng Chern Can Yuan Wenguan Wu Weiwei Ma Peng Qin Bintian Ma Xianjun Wu Shigui Li Pamela Ronald Xuewei Chen |
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| Affiliation: | 1. State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, China;2. Department of Plant Pathology and the Genome Center, University of California, Davis, CA, USA |
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| Abstract: | Receptor‐like cytoplasmic kinases (RLCKs) represent a large family of proteins in plants. However, few RLCKs have been well characterized. Here, we report the functional characterization of four rice RLCKs – OsRLCK57, OsRLCK107, OsRLCK118 and OsRLCK176 from subfamily VII. These OsRLCKs interact with the rice brassinosteroid receptor, OsBRI1 in yeast cell, but not the XA21 immune receptor. Transgenic lines silenced for each of these genes have enlarged leaf angles and are hypersensitive to brassinolide treatment compared to wild type rice. Transgenic plants silenced for OsRLCK57 had significantly fewer tillers and reduced panicle secondary branching, and lines silenced for OsRLCK107 and OsRLCK118 produce fewer seeds. Silencing of these genes decreased Xa21 gene expression and compromised XA21‐mediated immunity to Xanthomonas oryzae pv. oryzae. Our study demonstrates that these OsRLCKs negatively regulate BR signalling, while positively regulating immune responses by contributing to the expression of the immune receptor XA21. |
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| Keywords: | OsRLCK pattern recognition receptor |
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