High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector |
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| Authors: | Nazlina Ibrahim Malcolm A McCrae |
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| Institution: | (1) School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600 Selangor, Malaysia;(2) Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK |
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| Abstract: | Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen (HBcAg) as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20–37% of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5–6.4 mg l–1 of culture. |
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| Keywords: | Escherichia coli expression hepatitis B core antigen-VP4 fusion proteins |
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