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Expression of a Vibrio parahaemolyticus toxin in Escherichia coli results in chromosomal DNA degradation |
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| Authors: | Jing Zhang Saki Taniguchi Hironori Ito Kazuhiro Iiyama Madoka Hino Tsutomu Katayama |
| Affiliation: | 1. Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Fukuoka, Japan;2. Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan;3. Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University, Fukuoka, Japan;4. Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka, Japan;5. Department of Health and Nutrition Sciences, Faculty of Health and Social Welfare Science, Nishikyushu University, Saga, Japan |
| Abstract: | A toxin-antitoxin system, vp1842/vp1843, locates within a superintegron on the Vibrio parahaemolyticus genome chromosome I whose toxin gene vp1843 encodes a DNA nicking endonuclease. We found that the vp1843 expression in Escherichia coli cells strongly induced chromosomal DNA degradation. On the basis of these observations, we discuss a possible physiological role of vp1842/vp1843 in V. parahaemolyticus. |
| Keywords: | chromosomal DNA degradation toxin-antitoxin Vibrio parahaemolyticus |