3,4-Dihydroxyphenylacetic acid is a potential aldehyde dehydrogenase inducer in murine hepatoma Hepa1c1c7 cells |
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| Authors: | Yujia Liu Ayuki Kurita Sayaka Nakashima Beiwei Zhu Shintaro Munemasa Toshiyuki Nakamura |
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| Affiliation: | 1. Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan;2. School of Food Science and Technology, Dalian Polytechnic University, Dalian, China;3. School of Food Science and Technology, Dalian Polytechnic University, Dalian, China |
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| Abstract: | ![]()
3,4-Dihydroxyphenylacetic acid (DOPAC) is one of the major colonic microflora-produced catabolites of quercetin glycosides, such as quercetin 4′-glucoside derived from onion. Here, we investigated whether DOPAC modulates the aldehyde dehydrogenase (ALDH) activity and protects the cells from the acetaldehyde-induced cytotoxicity in vitro. DOPAC was shown to enhance not only the total ALDH activity, but also the gene expression of ALDH1A1, ALDH2 and ALDH3A1 in a concentration-dependent manner. DOPAC simultaneously stimulated the nuclear translocation of NFE2-related factor 2 and aryl hydrocarbon receptor. The pretreatment of DOPAC completely protected the cells from the acetaldehyde-induced cytotoxicity. The present study suggested that DOPAC acts as a potential ALDH inducer to prevent the alcohol-induced abnormal reaction. |
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| Keywords: | 3,4-dihydroxyphenylacetic acid aldehyde dehydrogenase acetaldehyde Nrf2 Hepa1c1c7 cells |
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