Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation |
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Authors: | Chen Huan Sun Yimin Dong Ruiqi Yang Shengsheng Pan Chuanyong Xiang Dao Miao Mingyong Jiao Binghua |
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Institution: | Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, China. |
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Abstract: | BackgroundMicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination.Methodology/Principal FindingMiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin βB (INHBB) and Met. In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver.Conclusion/SignificanceMiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34a-mediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation. |
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