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锌酵母中酵母甘露多糖组分的特征和结构
引用本文:张之申,董群.锌酵母中酵母甘露多糖组分的特征和结构[J].生物化学与生物物理学报,1995,27(6):651-656.
作者姓名:张之申  董群
作者单位:贵阳中医学院,中国科学院上海药物研究所
摘    要:本文研究从锌酵母中分离出的酵母甘露多糖XP的特征和结构。XP经全水解和^13CNMR谱显示除甘露糖基外,还有少量L-鼠李糖基和甲氧基。甲基化分析、过碘酸盐氧化、Smith降解、乙酰解和部分酸水解显示XP的主链是1→6连接的甘露糖,侧链是1→2连接的甘露糖。^1H及^13C NMR谱表明所有糖苷键均为α型,结合元素分析XP基本是酵母甘露多糖和蛋白质以及锌的络合物。

关 键 词:酵母  锌酵母  酵母多糖  甘露多糖

Studies on the Characteristics and Structure of Yeast Mannan from Zn-Containing Yeast
ZHANG Zhi-Shen, DONG Qun, FANG Ji-Nian, GUO Xi-Yong,LI Xiang-Dong and SHI Yu-Cheng.Studies on the Characteristics and Structure of Yeast Mannan from Zn-Containing Yeast[J].Acta Biochimica et Biophysica Sinica,1995,27(6):651-656.
Authors:ZHANG Zhi-Shen  DONG Qun  FANG Ji-Nian  GUO Xi-Yong  LI Xiang-Dong and SHI Yu-Cheng
Abstract:A yeast mannan XP from Zn-containing yeast was isolated and its characteristics and strueture were studied in detail. Completely hydrolyzed XP and the 13C NMR spectrum showed XP to contain a little L-rhemnose and O-methylated mannose besides mannose residues. Permethylation analysis, periodate oxidation,Smith degradation, acetolysis and partial acid bydrolysis revealed the main chains of XP as mannose of (1-6)-linkages and its side chains were mannose of (1-2)linkages. The 1H and 13C NMR spectrum indicated that all its glycosidic linkages are in the a-configuation. Elements analysis showed XP to be the complex of yeast mannan and protein and Zn.
Keywords:Zn-contained yeast  Zymosan  Mannan
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