|
|||||
|
|
| 胚胎发育早期转基因的PCR检测研究 | |
| 引用本文： | 卢一凡,邓继先,肖成祖,马清钧,王冬平. 胚胎发育早期转基因的PCR检测研究[J]. 中国实验动物学报, 1997, 0(1) |
| 作者姓名： | 卢一凡 邓继先 肖成祖 马清钧 王冬平 |
| 作者单位： | 军事医学科学院生物工程研究所，军事医学科学院动物中心 |
| 摘 要： | 转基因在受精卵中的整合时间对于转基因动物的建立十分重要。采用ＷＡＰ基因调控序列指导的人Ｇ－ＣＳＦ基因为构件，对小鼠受精卵进行显微注射。对培养至１细胞期、２细胞期和８细胞期的胚胎进行ＰＣＲ检测。结果表明，三个时期转基因的检出率分别为１００％、７７．７７％和４４．４４％。说明随着培养时间的增加，转基因逐渐丢失。 |
| 关 键 词： | 转基因胚 PCR 基因整合 |
Studies on Transgene with PCR Method in Early Embryo Development |
|
| Lu Yifan,et al. Studies on Transgene with PCR Method in Early Embryo Development[J]. Acta Laboratorium Animalis Scientia Sinica, 1997, 0(1) | |
| Authors: | Lu Yifan et al |
| Abstract: | Transgene was measured by using PCR method in early embryo development of fertilized eggs in this paper. 100%,77.7% and 44.4% retention of foreign gene were achieved in 1,2 and 88 cellstage resprectively. This result showed that transgene was missed in embryo development with time increasing. |
| Keywords: | Transgenic embryo PCR Gene integration |
| 本文献已被 CNKI 等数据库收录！ | |