Involvement of the cylindrical inclusion (CI) protein in the overcoming of an eIF4E-mediated resistance against Lettuce mosaic potyvirus |
| |
| Authors: | ANAS ABDUL-RAZZAK&dagger ,THOMAS GUIRAUD&dagger ,MARTINE PEYPELUT,JOCELYNE WALTER,MARIE-CHRISTINE HOUVENAGHEL,THIERRY CANDRESSE,OLIVIER LE GALL, SYLVIE GERMAN-RETANA |
| |
| Affiliation: | Interactions Plante-Virus, UMR GDPP 1090, INRA Universitéde Bordeaux 2, BP 81, F-33883 Villenave d'Ornon, France |
| |
| Abstract: | The capacity of Lettuce mosaic virus to overcome the lettuce resistance conferred by the mo11 and mo12 alleles of the gene for eukaryotic translation initiation factor 4E (eIF4E) was analysed using reverse genetics. Mutations in the virus genome-linked protein (VPg) allowed mo11 only to be overcome, but mutations in the C-terminal portion of the cylindrical inclusion (CI) protein allowed both alleles to be overcome. Site-directed mutagenesis pinpointed a key role of the amino acid at position 621 in the virulence. This is the first example of the involvement of a potyviral CI protein in the breaking of an eIF4E-mediated resistance. |
| |
| Keywords: | |
|
|
