Solid state cultivation of Streptomyces clavuligerus for cephamycin C production |
| |
Institution: | 1. Department of Veterinary Parasitology and Entomology, University of Nigeria, Nsukka, Nigeria;2. Department of Animal Health and Production, Nigeria |
| |
Abstract: | Solid state cultivation of Streptomyces clavuligerus for cephamycin C production was carried out in a system consisting of wheat rawa 5 g; cotton seed deoiled cake 5 g; sunflower cake 0·5 g; corn steep liquor 1 g; MgSO4.7H2O 0·06 g; CaCO3 0·1 g; K2HPO4 4·4 g; with initial moisture content of 80%, initial pH 6·5 and a fermentation temperature in the range 28–30°C. The fermentation cycle was about 5 days. Streptomyces clavuligerus growth was observed on the 2nd day and production of cephamycin C was initiated on 3rd day. Abundant mycelial growth was observed from the 3rd day and reached stationary phase by the 5th day. Cephamycin C was produced maximally at a rate of 15 mg/g substrate on the 5th day and was stable until the 30th day with only marginal decrease in titre. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|